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KMID : 0545120090190030265
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Journal of Microbiology and Biotechnology
2009 Volume.19 No. 3 p.265 ~ p.270
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Oligonucleotide Array-based Detection and Genotyping of Mollicutes (Acholeplasma, Mycoplasma, and Ureaplasma)
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Jang Hyun-Jung
Kim Hyo-myeung
Kang Byeong-Chul
Kim Cheol-Min
Park Hee-Kyung
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Abstract
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An oligonucleotide array was developed to detect and genotype mollicutes based on the internal transcribed spacer (ITS) sequence. The results of the assay were compared with those of a PCR-RFLP assay. The proposed oligonucleotide array containing 5 genus- and 23 species-specific probes was able to detect Mycoplasma species, including M. penetrans and M. spermatophilum, that were not detected by the PCRRFLP assay. Therefore, the results demonstrated that the proposed oligonucleotide array was effective for the detection and discrimination of 23 species, including an acholeplasma, 21 mycoplasmas, and a ureaplasma, and showed promise as a countermeasure to ensure that biological products are safe and of good quality.
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KEYWORD
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Oligonucleotide array, mollicute, internal transcribed spacer, genotyping
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